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miscript ii reverse transcriptase  (Qiagen)


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    Structured Review

    Qiagen miscript ii reverse transcriptase
    Miscript Ii Reverse Transcriptase, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 6786 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miscript+ii+reverse+transcriptase/Reverse+transcriptase/pmc13044240-116-17-21
    Average 97 stars, based on 6786 article reviews
    miscript ii reverse transcriptase - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: Monascus red pigment influence on hydroxyapatite nanoparticles-mediated renal toxicity in rats
    Article Snippet: The caspase-3 enzymatic activity was assayed using Caspase-3 Assay Kit from Elabscience (USA) in compliance with the manufacturer’s protocol. .. Kidney total RNA was isolated using RNeasy mini kit (Qiagen, Hilden, Germany) and reverse transcribed into cDNA using miScript II reverse transcriptase (Qiagen, Hilden, Germany). .. Real-time PCR using specific primer sets for Kim-1 (Accession No NM_173149.2; sense: TGG CAC TGT GAC ATC CTC AGA, antisense: GCA ACG GAC ATG CCA ACA TA) and lipocalin-2 (Accession No NM_130741.1; sense: GGA ATA TTC ACA GCT ACC CTC, antisense: TTG TTA TCC TTG AGG CCC AG] was performed using SYBR green (Qiagen, Hilden, Germany) and 18s rRNA (Accession No NR_046237.2; sense primer: GTA ACC CGT TGA ACC CCA TT; antisense primer: CAA GCT TAT GAC CCG CAC TT) as a reference gene in a LightCycler machine (Roche, Basel, Switzerland).

    Article Title: Protective role of Monascus red pigment against hydroxyapatite nanoparticle-induced liver injury in rats via modulation of metabolic regulators.
    Article Snippet: Hydroxyapatite nanoparticles (HANPs) are widely used in biomedical applications due to their biocompatibility and structural similarity to human bones.. However, like other nanoparticles, HANPs circulate in the bloodstream and accumulate in organs such as the liver, raising toxicity concerns.. This study investigated the hepatic effects of oral HANPs exposure at a dose of 88.3 mg/kg body weight in male rats and evaluated the protective potential of varying doses of Monascus purpureus red pigment (RP; 10, 20, 40 mg/kg body weight).

    Article Title: Experimental and molecular docking evidence for the protective role of Monascus purpureus red pigments against hydroxyapatite nanoparticle-induced testicular injury in male rats
    Article Snippet: .. Total RNA was isolated using RNeasy mini kit (Qiagen, Hilden, Germany) and reverse transcribed into cDNA using miScript II reverse transcriptase (Qiagen, Hilden, Germany). .. Real-time PCR using specific primer sets for Beclin-1 (Accession No NM_001034117.1 ; sense: TTG GCC AAT AAG ATG GGT CTG AA, antisense: TGT CAG GGA CTC CAG ATA CGA GTG), LC3B (Accession No NM_022867.2 ; sense: CAG GAT CCA TGC CGT CCC AGA AGA CC, antisense: GTC CCT TTT TGC CTT GGT AG), ULK1 (Accession No NM_001108341.1 ; sense: CAT CCG AAG GTC AGG TAG CA, antisense: GAT GGT TCC CAC TTG GGG AGA), and ATG9 (Accession No NM_001014218 ; sense: CCC ACG GGC CCT GGA GAT CA, antisense: CCG TGC TGG CGA ACG TCC AT), was performed using SYBR green (Qiagen, Hilden, Germany) and 18s rRNA (Accession No NR_046237.2 ; sense primer: GTA ACC CGT TGA ACC CCA TT; antisense primer: CAA GCT TAT GAC CCG CAC TT) as a reference gene in a LightCycler machine (Roche, Basel, Switzerland).

    Article Title: Experimental and molecular docking evidence for the protective role of Monascus purpureus red pigments against hydroxyapatite nanoparticle-induced testicular injury in male rats.
    Article Snippet: .. Expression analysis of key autophagy-related genes using quantitative real time-polymerase chain reaction (qRT-PCR) Total RNA was isolated using RNeasy mini kit (Qiagen, Hilden, Germany) and reverse transcribed into cDNA using miScript II reverse transcriptase (Qiagen, Hilden, Germany). .. Real-time PCR using specific primer sets for Beclin-1 (Accession No NM_001034117.1; sense: TTG GCC AAT AAG ATG GGT CTG AA, antisense: TGT CAG GGA CTC CAG ATA CGA GTG), LC3B (Accession No NM_022867.2; sense: CAG GAT CCA TGC CGT CCC AGA AGA CC, antisense: GTC CCT TTT TGC CTT GGT AG), ULK1 (Accession No NM_001108341.1; sense: CAT CCG AAG GTC AGG TAG CA, antisense: GAT GGT TCC CAC TTG GGG AGA), and ATG9 (Accession No NM_001014218; sense: CCC ACG GGC CCT GGA GAT CA, antisense: CCG TGC TGG CGA ACG TCC AT), was performed using SYBR green (Qiagen, Hilden, Germany) and 18s rRNA (Accession No NR_046237.2; sense primer: GTA ACC CGT TGA ACC CCA TT; antisense primer: CAA GCT TAT GAC CCG CAC TT) as a reference gene in a LightCycler machine (Roche, Basel, Switzerland).

    Reverse Transcription:

    Article Title: Monascus red pigment influence on hydroxyapatite nanoparticles-mediated renal toxicity in rats
    Article Snippet: The caspase-3 enzymatic activity was assayed using Caspase-3 Assay Kit from Elabscience (USA) in compliance with the manufacturer’s protocol. .. Kidney total RNA was isolated using RNeasy mini kit (Qiagen, Hilden, Germany) and reverse transcribed into cDNA using miScript II reverse transcriptase (Qiagen, Hilden, Germany). .. Real-time PCR using specific primer sets for Kim-1 (Accession No NM_173149.2; sense: TGG CAC TGT GAC ATC CTC AGA, antisense: GCA ACG GAC ATG CCA ACA TA) and lipocalin-2 (Accession No NM_130741.1; sense: GGA ATA TTC ACA GCT ACC CTC, antisense: TTG TTA TCC TTG AGG CCC AG] was performed using SYBR green (Qiagen, Hilden, Germany) and 18s rRNA (Accession No NR_046237.2; sense primer: GTA ACC CGT TGA ACC CCA TT; antisense primer: CAA GCT TAT GAC CCG CAC TT) as a reference gene in a LightCycler machine (Roche, Basel, Switzerland).

    Article Title: Expression of microRNAs and their target genes and pathways associated with ovarian follicle development in cattle.
    Article Snippet: doi:10.1152/physiolgenomics.00036.2014 46:735-745, 2014.. First published 5 August 2014;Physiol.. Genomics T. Szulc and A. C. O. Evans A. E. Zielak-Steciwko, J.

    Article Title: Protective role of Monascus red pigment against hydroxyapatite nanoparticle-induced liver injury in rats via modulation of metabolic regulators.
    Article Snippet: Hydroxyapatite nanoparticles (HANPs) are widely used in biomedical applications due to their biocompatibility and structural similarity to human bones.. However, like other nanoparticles, HANPs circulate in the bloodstream and accumulate in organs such as the liver, raising toxicity concerns.. This study investigated the hepatic effects of oral HANPs exposure at a dose of 88.3 mg/kg body weight in male rats and evaluated the protective potential of varying doses of Monascus purpureus red pigment (RP; 10, 20, 40 mg/kg body weight).

    Article Title: Experimental and molecular docking evidence for the protective role of Monascus purpureus red pigments against hydroxyapatite nanoparticle-induced testicular injury in male rats
    Article Snippet: .. Total RNA was isolated using RNeasy mini kit (Qiagen, Hilden, Germany) and reverse transcribed into cDNA using miScript II reverse transcriptase (Qiagen, Hilden, Germany). .. Real-time PCR using specific primer sets for Beclin-1 (Accession No NM_001034117.1 ; sense: TTG GCC AAT AAG ATG GGT CTG AA, antisense: TGT CAG GGA CTC CAG ATA CGA GTG), LC3B (Accession No NM_022867.2 ; sense: CAG GAT CCA TGC CGT CCC AGA AGA CC, antisense: GTC CCT TTT TGC CTT GGT AG), ULK1 (Accession No NM_001108341.1 ; sense: CAT CCG AAG GTC AGG TAG CA, antisense: GAT GGT TCC CAC TTG GGG AGA), and ATG9 (Accession No NM_001014218 ; sense: CCC ACG GGC CCT GGA GAT CA, antisense: CCG TGC TGG CGA ACG TCC AT), was performed using SYBR green (Qiagen, Hilden, Germany) and 18s rRNA (Accession No NR_046237.2 ; sense primer: GTA ACC CGT TGA ACC CCA TT; antisense primer: CAA GCT TAT GAC CCG CAC TT) as a reference gene in a LightCycler machine (Roche, Basel, Switzerland).

    Article Title: Regulation of Ras homolog family member G by microRNA-124 regulates proliferation and migration of human retinal pigment epithelial cells
    Article Snippet: The culture medium was aspirated and cells were rinsed thrice with phosphate-buffered saline (PBS); thereafter, total RNA was extracted using QIAzol lysis reagent (Qiagen, Hilden, Germany). .. A 1-μg aliquot of total RNA was reverse transcribed using miScript II Reverse Transcriptase (Qiagen, Hilden, Germany). qPCR was performed using QuantiTect® SYBR® Green PCR Master Mix and the Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA). ..

    Article Title: The micro RNA hsa-miR-377-3p inhibits tumor growth in malignant melanoma
    Article Snippet: Total RNA was extracted using Trizol reagent (Invitrogen, 15596- 018, USA) from the A375 and HEMa-LP cell lines. .. RNA quantication and quality were measured by NanoDrop ND-2000 (Thermo, USA). cDNA synthesis for mciroRNA was performed using miScript II Reverse Transcriptase (Qiagen). ..

    Article Title: Experimental and molecular docking evidence for the protective role of Monascus purpureus red pigments against hydroxyapatite nanoparticle-induced testicular injury in male rats.
    Article Snippet: .. Expression analysis of key autophagy-related genes using quantitative real time-polymerase chain reaction (qRT-PCR) Total RNA was isolated using RNeasy mini kit (Qiagen, Hilden, Germany) and reverse transcribed into cDNA using miScript II reverse transcriptase (Qiagen, Hilden, Germany). .. Real-time PCR using specific primer sets for Beclin-1 (Accession No NM_001034117.1; sense: TTG GCC AAT AAG ATG GGT CTG AA, antisense: TGT CAG GGA CTC CAG ATA CGA GTG), LC3B (Accession No NM_022867.2; sense: CAG GAT CCA TGC CGT CCC AGA AGA CC, antisense: GTC CCT TTT TGC CTT GGT AG), ULK1 (Accession No NM_001108341.1; sense: CAT CCG AAG GTC AGG TAG CA, antisense: GAT GGT TCC CAC TTG GGG AGA), and ATG9 (Accession No NM_001014218; sense: CCC ACG GGC CCT GGA GAT CA, antisense: CCG TGC TGG CGA ACG TCC AT), was performed using SYBR green (Qiagen, Hilden, Germany) and 18s rRNA (Accession No NR_046237.2; sense primer: GTA ACC CGT TGA ACC CCA TT; antisense primer: CAA GCT TAT GAC CCG CAC TT) as a reference gene in a LightCycler machine (Roche, Basel, Switzerland).

    Article Title: The micro RNA hsa-miR-377-3p inhibits tumor growth in malignant melanoma
    Article Snippet: Total RNA was extracted using Trizol reagent (Invitrogen, 15596-018, USA) from the A375 and HEMa-LP cell lines. .. RNA quantification and quality were measured by NanoDrop ND-2000 (Thermo, USA). cDNA synthesis for mciroRNA was performed using miScript II Reverse Transcriptase (Qiagen). ..

    Quantitative RT-PCR:

    Article Title: Expression of microRNAs and their target genes and pathways associated with ovarian follicle development in cattle.
    Article Snippet: doi:10.1152/physiolgenomics.00036.2014 46:735-745, 2014.. First published 5 August 2014;Physiol.. Genomics T. Szulc and A. C. O. Evans A. E. Zielak-Steciwko, J.

    Real-time Polymerase Chain Reaction:

    Article Title: Expression of microRNAs and their target genes and pathways associated with ovarian follicle development in cattle.
    Article Snippet: doi:10.1152/physiolgenomics.00036.2014 46:735-745, 2014.. First published 5 August 2014;Physiol.. Genomics T. Szulc and A. C. O. Evans A. E. Zielak-Steciwko, J.

    Article Title: Regulation of Ras homolog family member G by microRNA-124 regulates proliferation and migration of human retinal pigment epithelial cells
    Article Snippet: The culture medium was aspirated and cells were rinsed thrice with phosphate-buffered saline (PBS); thereafter, total RNA was extracted using QIAzol lysis reagent (Qiagen, Hilden, Germany). .. A 1-μg aliquot of total RNA was reverse transcribed using miScript II Reverse Transcriptase (Qiagen, Hilden, Germany). qPCR was performed using QuantiTect® SYBR® Green PCR Master Mix and the Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA). ..

    Article Title: Experimental and molecular docking evidence for the protective role of Monascus purpureus red pigments against hydroxyapatite nanoparticle-induced testicular injury in male rats.
    Article Snippet: .. Expression analysis of key autophagy-related genes using quantitative real time-polymerase chain reaction (qRT-PCR) Total RNA was isolated using RNeasy mini kit (Qiagen, Hilden, Germany) and reverse transcribed into cDNA using miScript II reverse transcriptase (Qiagen, Hilden, Germany). .. Real-time PCR using specific primer sets for Beclin-1 (Accession No NM_001034117.1; sense: TTG GCC AAT AAG ATG GGT CTG AA, antisense: TGT CAG GGA CTC CAG ATA CGA GTG), LC3B (Accession No NM_022867.2; sense: CAG GAT CCA TGC CGT CCC AGA AGA CC, antisense: GTC CCT TTT TGC CTT GGT AG), ULK1 (Accession No NM_001108341.1; sense: CAT CCG AAG GTC AGG TAG CA, antisense: GAT GGT TCC CAC TTG GGG AGA), and ATG9 (Accession No NM_001014218; sense: CCC ACG GGC CCT GGA GAT CA, antisense: CCG TGC TGG CGA ACG TCC AT), was performed using SYBR green (Qiagen, Hilden, Germany) and 18s rRNA (Accession No NR_046237.2; sense primer: GTA ACC CGT TGA ACC CCA TT; antisense primer: CAA GCT TAT GAC CCG CAC TT) as a reference gene in a LightCycler machine (Roche, Basel, Switzerland).

    Polymerase Chain Reaction:

    Article Title: Expression of microRNAs and their target genes and pathways associated with ovarian follicle development in cattle.
    Article Snippet: doi:10.1152/physiolgenomics.00036.2014 46:735-745, 2014.. First published 5 August 2014;Physiol.. Genomics T. Szulc and A. C. O. Evans A. E. Zielak-Steciwko, J.

    Article Title: Regulation of Ras homolog family member G by microRNA-124 regulates proliferation and migration of human retinal pigment epithelial cells
    Article Snippet: The culture medium was aspirated and cells were rinsed thrice with phosphate-buffered saline (PBS); thereafter, total RNA was extracted using QIAzol lysis reagent (Qiagen, Hilden, Germany). .. A 1-μg aliquot of total RNA was reverse transcribed using miScript II Reverse Transcriptase (Qiagen, Hilden, Germany). qPCR was performed using QuantiTect® SYBR® Green PCR Master Mix and the Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA). ..

    SYBR Green Assay:

    Article Title: Regulation of Ras homolog family member G by microRNA-124 regulates proliferation and migration of human retinal pigment epithelial cells
    Article Snippet: The culture medium was aspirated and cells were rinsed thrice with phosphate-buffered saline (PBS); thereafter, total RNA was extracted using QIAzol lysis reagent (Qiagen, Hilden, Germany). .. A 1-μg aliquot of total RNA was reverse transcribed using miScript II Reverse Transcriptase (Qiagen, Hilden, Germany). qPCR was performed using QuantiTect® SYBR® Green PCR Master Mix and the Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA). ..

    cDNA Synthesis:

    Article Title: The micro RNA hsa-miR-377-3p inhibits tumor growth in malignant melanoma
    Article Snippet: Total RNA was extracted using Trizol reagent (Invitrogen, 15596- 018, USA) from the A375 and HEMa-LP cell lines. .. RNA quantication and quality were measured by NanoDrop ND-2000 (Thermo, USA). cDNA synthesis for mciroRNA was performed using miScript II Reverse Transcriptase (Qiagen). ..

    Article Title: The micro RNA hsa-miR-377-3p inhibits tumor growth in malignant melanoma
    Article Snippet: Total RNA was extracted using Trizol reagent (Invitrogen, 15596-018, USA) from the A375 and HEMa-LP cell lines. .. RNA quantification and quality were measured by NanoDrop ND-2000 (Thermo, USA). cDNA synthesis for mciroRNA was performed using miScript II Reverse Transcriptase (Qiagen). ..

    Expressing:

    Article Title: Experimental and molecular docking evidence for the protective role of Monascus purpureus red pigments against hydroxyapatite nanoparticle-induced testicular injury in male rats.
    Article Snippet: .. Expression analysis of key autophagy-related genes using quantitative real time-polymerase chain reaction (qRT-PCR) Total RNA was isolated using RNeasy mini kit (Qiagen, Hilden, Germany) and reverse transcribed into cDNA using miScript II reverse transcriptase (Qiagen, Hilden, Germany). .. Real-time PCR using specific primer sets for Beclin-1 (Accession No NM_001034117.1; sense: TTG GCC AAT AAG ATG GGT CTG AA, antisense: TGT CAG GGA CTC CAG ATA CGA GTG), LC3B (Accession No NM_022867.2; sense: CAG GAT CCA TGC CGT CCC AGA AGA CC, antisense: GTC CCT TTT TGC CTT GGT AG), ULK1 (Accession No NM_001108341.1; sense: CAT CCG AAG GTC AGG TAG CA, antisense: GAT GGT TCC CAC TTG GGG AGA), and ATG9 (Accession No NM_001014218; sense: CCC ACG GGC CCT GGA GAT CA, antisense: CCG TGC TGG CGA ACG TCC AT), was performed using SYBR green (Qiagen, Hilden, Germany) and 18s rRNA (Accession No NR_046237.2; sense primer: GTA ACC CGT TGA ACC CCA TT; antisense primer: CAA GCT TAT GAC CCG CAC TT) as a reference gene in a LightCycler machine (Roche, Basel, Switzerland).



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